Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics
نویسندگان
چکیده
منابع مشابه
Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics
Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research, pathology and drug discovery and development. With the rapid growth of our understanding of gene and protein expression pathways and limited supplies of tissue, there is an ever-increasing need for more s...
متن کاملBioconjugation of quantum dot luminescent probes for Western blot analysis.
Western blot analysis is a widely used technique for protein immunodetection. Its current format, however is unsuitable for multiplex detection of proteins, primarily due to intrinsic limitations of standard organic dyes employed as probes. Quantum dot (QD) semiconductor nanoparticles exhibit significant advantages over organic dyes, including their broad absorption bands, narrow, tunable, and ...
متن کاملQuantitative, Two-Color Western Blot Detection With Infrared Fluorescence
Accurate and reproducible quantifi cation of proteins on Western blots across a wide linear range is desirable, but diffi cult to achieve. Chemiluminescent detection is subject to enzyme/substrate kinetics that may affect performance, and use of antibodies labeled with visible fl uorophores has not provided the necessary sensitivity for practical use. In this study, we investigated the use of a...
متن کاملQuantum dot-based multiplexed fluorescence resonance energy transfer.
We demonstrate the use of luminescent quantum dots (QDs) conjugated to dye-labeled protein acceptors for nonradiative energy transfer in a multiplexed format. Two configurations were explored: (1) a single color QD interacting with multiple distinct acceptors and (2) multiple donor populations interacting with one type of acceptor. In both cases, we showed that simultaneous energy transfer betw...
متن کاملQuantitative dot blot analysis (QDB), a versatile high throughput immunoblot method
Lacking access to an affordable method of high throughput immunoblot analysis for daily use remains a big challenge for scientists worldwide. We proposed here Quantitative Dot Blot analysis (QDB) to meet this demand. With the defined linear range, QDB analysis fundamentally transforms traditional immunoblot method into a true quantitative assay. Its convenience in analyzing large number of samp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2005
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0105-79